Customization: | Available |
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CAS No.: | 75621-03-3 |
Formula: | C32h58n2o7s |
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Description:
CHAPS is a white crystalline powder that it is structurally similar to certain bile acids, such as taurodeoxycholic acid and taurochenodeoxycholic acid. It is sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity. Chaps may be synthesized from cholic acid and is zwitterionic due to its quaternary ammonium and sulfonate groups.
Application:
CHAPS is often used as a detergent in the solubilization and purification of membrane proteins for several advantageous reasons. CHAPS detergent is non-denaturing to membrane proteins, can solubilize proteins, disaggregate protein-protein interactions. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH 2 to 12. It is suitable for isolation of membrane proteins with the following features such as sufficient protein solubilization capability, no denaturing or inactivation of proteins, no interference with protein activities, no precipitation at 4°C, appropriate critical micelle concentrations (CMC) and micelle size, no absorption in the UV region, no toxicity and availability of detergent detection methods.
CAS Number | 75621-03-3 |
Molecular Formula | C32H58N2O7S |
Molecular Weight | 614.88 |
EINECS | 616-246-2 |
Appearance | White Powder |
Density | 1.01g/cm3 |
Melting Point | 156-158ºC |
Solubility | H2O: 50 mg/mL at 20 °C, clear, colorless |
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